multi-function microplate reader tecan infinite® 200 pro  (Tecan Systems)

Journal: Oncology Letters

Article Title: Luteolin induces apoptosis by impairing mitochondrial function and targeting the intrinsic apoptosis pathway in gastric cancer cells

doi: 10.3892/ol.2023.13913

Figure Lengend Snippet: ROS accumulation in HGC-27 and MFC cells is induced by luteolin. The fluorescence intensity of (A) HGC-27 cells and (B) MFC cells were visualized under a fluorescence inverted microscope (magnification, ×100). Luteolin-induced ROS levels were detected using DCFH-DA staining and flow cytometry in (C) HGC-27 cells and (E) MFC cells. The quantitative analysis of ROS levels in (D) HGC-27 cells and (F) MFC cells was showed on the histogram. (G) SOD activity was assessed in HGC-27 and MFC cells using a microplate reader at an absorbance of 560 nm. Experiments were repeated at least in triplicate. Data were presented as mean ± SD. **P<0.01 vs. DMSO group. ROS, reactive oxygen species; SOD, superoxide dismutase; HGC-27, human gastric cancer HGC-27 cell line; MFC, mouse forestomach carcinoma cell line.

Article Snippet: After incubation at 37°C for 60 min in the dark, the absorbance at a wavelength of 450 nm was detected using a Thermo 3001 multi-function microplate reader (Infinite 200 PRO, Tecan Austria GmbH, Salzburg, Austria).

Techniques: Fluorescence, Inverted Microscopy, Staining, Flow Cytometry, Activity Assay

Journal: Oncology Letters

Article Title: Luteolin induces apoptosis by impairing mitochondrial function and targeting the intrinsic apoptosis pathway in gastric cancer cells

doi: 10.3892/ol.2023.13913

Figure Lengend Snippet: Mitochondrial membrane potential, ATP levels and some enzyme activities in HGC-27 and MFC cells induced by luteolin. The luteolin-treated (A) HGC-27 cells and (C) MFC cells were stained with JC-1, and analyzed using flow cytometry. Quantitative statistics of the mitochondrial membrane potential was based on flow cytometry in (B) HGC-27 cells and (D) MFC cells. The (E) ATP levels, (F) Na + /K + -ATPase activities and (G) Ca 2+ /Mg 2+ -ATPase activities were showed based on the microplate system at the absorbance values of 660 nm. The data were presented as mean ± SD. The experiments were repeated in triplicate. *P<0.05, **P<0.01 vs. DMSO group. HGC-27, human gastric cancer HGC-27 cell line; MFC, mouse forestomach carcinoma cell line.

Article Snippet: After incubation at 37°C for 60 min in the dark, the absorbance at a wavelength of 450 nm was detected using a Thermo 3001 multi-function microplate reader (Infinite 200 PRO, Tecan Austria GmbH, Salzburg, Austria).

Techniques: Membrane, Staining, Flow Cytometry

Journal: Oncology Letters

Article Title: Luteolin induces apoptosis by impairing mitochondrial function and targeting the intrinsic apoptosis pathway in gastric cancer cells

doi: 10.3892/ol.2023.13913

Figure Lengend Snippet: Luteolin induces the enzyme activities of the METC complexes in HGC-27 and MFC cells. Complexes (A) I, (B) III and (C) V were assessed by testing kits. The data were obtained using a microplate reader at 340, 550 and 660 nm, respectively. The data were presented as mean ± SD. The experiments were performed at least in triplicate. *P<0.05 and **P<0.01 vs. DMSO group; HGC-27, human gastric cancer HGC-27 cell line; MFC, mouse forestomach carcinoma cell line.

Article Snippet: After incubation at 37°C for 60 min in the dark, the absorbance at a wavelength of 450 nm was detected using a Thermo 3001 multi-function microplate reader (Infinite 200 PRO, Tecan Austria GmbH, Salzburg, Austria).

Techniques: